A thermostable transketolase evolved for aliphatic aldehyde acceptors.
نویسندگان
چکیده
Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 3 شماره
صفحات -
تاریخ انتشار 2015